perk 1 2 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
perk 1 2
Perk 1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perk+1+2/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pm41786829-102-78-80
Average 99 stars, based on 16813 article reviews
Perk 1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perk+1+2/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pm41786829-102-78-80
Average 99 stars, based on 16813 article reviews
perk 1 2 - by Bioz Stars,
2026-09
99/100 stars
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Western Blot:Article Title: Targeting Triple-Negative Breast Cancer with Momordicine-I for Therapeutic Gain in Preclinical Models. Article Snippet: .. Cell lysates were prepared using 2XSDS sample buffer, and Western blot analysis was performed using specific antibodies to Cyclin D1 (1:500, Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-20044, RRID:AB_627346), c-Myc (1:1000, Cell Signaling Technology, Danvers, MA, USA, CST-18583, RRID:AB_2895543), pSTAT3 (1:1000, Cell Signaling Technology, CST-9145 (Tyr705) (D3A7) RRID:AB_2491009), STAT3 (1:1000, Cell Signaling Technology, CST-30835 (D1B2), RRID:AB_2798995), Article Title: Targeting Triple-Negative Breast Cancer with Momordicine-I for Therapeutic Gain in Preclinical Models Article Snippet: .. Cell lysates were prepared using 2XSDS sample buffer, and Western blot analysis was performed using specific antibodies to Cyclin D1 (1:500, Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-20044, RRID:AB_627346), c-Myc (1:1000, Cell Signaling Technology, Danvers, MA, USA, CST-18583, RRID:AB_2895543), pSTAT3 (1:1000, Cell Signaling Technology, CST-9145 (Tyr705) (D3A7) RRID:AB_2491009), STAT3 (1:1000, Cell Signaling Technology, CST-30835 (D1B2), RRID:AB_2798995), Article Title: KRAS-mediated upregulation of CIP2A promotes suppression of PP2A-B56α to initiate pancreatic cancer development. Article Snippet: Membranes were then incubated with secondary antibody for 1H at RT and scanned using the Licor Odyssey DLx Imaging system and analyzed using Image Studio software. .. Antibodies used for Western blot: CIP2A (KIAA1524, HPA039570, lot 000008181, Atlas Biosciences), CIP2A (sc-80659, lot H2522, Santa Cruz Biotechnologies), B56α (Clone 3A6D3, Egon Ogris Lab), MYC (ab32072, lot GR3377350-8-YR091119PS, Abcam), GAPDH (M4300, lot 820845, ThermoFisher), Article Title: Metabolic STAMP for deciphering GPCR-regulated insulin secretion by pancreatic β cells Article Snippet: For IF : acetylated tubulin (mouse IgG2B; Santa Cruz; Cat #: SC-23950; 1:2000 of 200 μg/ml stock), L-tubulin (mouse IgG1; Sigma-Aldrich; Cat #: T5201), insulin (guinea pig; discontinued antibody gifted from Seung Kim Lab; , 1:500 of stock (mouse IgG1; Sigma-Aldrich; Cat #: T5201; 1:2000 of 2mg/mL stock), GM130 (mouse IgG1; BD Biosciences; Cat #: 610823; 1:500 of 250μg/mL), L-COP (rabbit; gifted from Richard Kahn lab; 1:1000 of stock), HDAC6 (Rabbit; US Biologicals; Cat #: H1827-66; 1:250 of 0.1mg/mL stock), ATAT1 (Rabbit; Millipore-Sigma; Cat #: HPA046816; 1:250 dilution of 0.1mg/mL stock), Complex I (mouse Ig2B; Thermofisher; Cat #:43-8800; 1:1000 of 1mg/mL stock). .. For Western blot : Saline:Article Title: Causal relevance of the JAK/STAT pathway for cardioprotection via cold acclimation. Article Snippet: Using a Wet Blot Module (Bio-Rad), proteins were electro-transferred onto nitrocellulose membranes (0.2–0.45 μm pore size; Bio-Rad) at 100 V for 90 min 10,11,26. .. Membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.05% Tween 20 (20 mM Tris, 500 mM NaCl) for 1 h and incubated overnight at 4 °C with polyclonal antibodies against STAT3 (CS30835; Cell Signaling Technology), pSTAT3-Y705 (CS4113), pSTAT3-S727 (Ab32143; Abcam), Bcl-2 (SAB4500003), BAX (Ab7977), Caspase-3 (Ab13847), p38 (sc-SC535; Santa Cruz), p-p38 (sc-17852), p-p38-T180/Y182 (sc-17852-R), Akt (SC8312), p-Akt-S473 (CS9271); MEK-1/2 (cs-9122, Cell Signaling Technology), pMEK-1/2-S217/221 (cs-9121, Cell Signaling Technology), ERK-1/2 (cs-9102, Cell Signaling Technology), Incubation:Article Title: Causal relevance of the JAK/STAT pathway for cardioprotection via cold acclimation. Article Snippet: Using a Wet Blot Module (Bio-Rad), proteins were electro-transferred onto nitrocellulose membranes (0.2–0.45 μm pore size; Bio-Rad) at 100 V for 90 min 10,11,26. .. Membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.05% Tween 20 (20 mM Tris, 500 mM NaCl) for 1 h and incubated overnight at 4 °C with polyclonal antibodies against STAT3 (CS30835; Cell Signaling Technology), pSTAT3-Y705 (CS4113), pSTAT3-S727 (Ab32143; Abcam), Bcl-2 (SAB4500003), BAX (Ab7977), Caspase-3 (Ab13847), p38 (sc-SC535; Santa Cruz), p-p38 (sc-17852), p-p38-T180/Y182 (sc-17852-R), Akt (SC8312), p-Akt-S473 (CS9271); MEK-1/2 (cs-9122, Cell Signaling Technology), pMEK-1/2-S217/221 (cs-9121, Cell Signaling Technology), ERK-1/2 (cs-9102, Cell Signaling Technology), |